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Pneumoniae infection, antibiotic exposure

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antibiotics penicillin alcohol cells were lysed, and the remaining white blood cells

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were assessed by flow cytometry

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with gating on PMNs. Effect of antibiotics on polymorphonuclear

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neutrophil apoptosis.STUDY

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OBJECTIVE. Pneumoniae, all antibiotic treatments, even those with poor in vitro activity for the bacterial isolate,

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decreased PMN apoptosis by 26.2% (range -38.0% to -17.8%, p. To evaluate the effects of various antibiotics-direct and

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indirect as a result of bacterial killing-on polymorphonuclear neutrophil (PMN) apoptosis. Whole blood collected from healthy human subjects was incubated with and without Klebsiella pneumoniae (1.0 x 10(5) colony-forming units [cfu]/ml) plus ceftazidime 50 microg/ml, gentamicin, ciprofloxacin,

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trovafloxacin, tetracycline, doxycycline, erythromycin, azithromycin ( Zithromax ) (each 5 microg/ml), or lipopolysaccharide 10 microg/ml.